LOCUS       TGEX-HC-hG4[S228        5819 bp    DNA     circular
DEFINITION  Human IgG4[S228P] TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3127..3715,3731..3829)
            FT /number="17"
            FT color
            FT join(1100..1103,5579..5607)
            FT /number="1"
            FT color
            FT 2744..3118
            FT /number="20"
            FT color
            FT join(1041..1099,1128..1469,1472..1751,1754..2114)
            FT /number="14"
            FT translated
            FT 2744..3118
            FT /frame="2"
            FT color
            FT 3903..4522
            FT /number="2"
            FT color
            FT join(2137..2147,2150..2649,2651,2654..2710)
            FT /number="7"
            FT color
            FT join(4677..4817,4819..5537)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
            FT translated
            FT 1128..2112
            FT /frame="3"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2111
                     /codon_start=1
                     /translation="ASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSG
                     ALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKY
                     GPPCPPCPAPEFEGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVD
                     GVEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLGSSIEKTISK
                     AKGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPP
                     VLDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK*"
                     /gene
                     /label=hG4*01
                     /note="S228P"
     misc_difference 1449..1451
                     /label=S228P
                     /note="S>P [TCA -> CCT] "
     misc_binding    complement(2081..2087)
                     /db_xref="REBASE:SapI"
     misc_feature    2109..2114
                     /gene
                     /label=Stop
                     /note
     misc_binding    2115..2120
                     /db_xref="REBASE:NheI"
     misc_feature    2137..2710
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2183..2202)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2647..2653
                     /label=A7
                     /note
     misc_binding    2732..2737
                     /db_xref="REBASE:XbaI"
     misc_binding    2738..2743
                     /db_xref="REBASE:SalI"
     CDS             2744..3118
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3119..3126
                     /db_xref="REBASE:NotI"
     misc_feature    3127..3715
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3731..3829
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3846..3851
                     /db_xref="REBASE:BglII"
     misc_binding    3852..3857
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3903..4522)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4359)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4677..5537)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5579..5607)
                     /label=AmpR_Promoter
     misc_binding    5670..5675
                     /db_xref="REBASE:BglII"
     misc_difference 1470..1471
                     /gene
                     /label=L235E
                     /note
     misc_feature    1752..1753
                     /gene
                     /label=P329G
                     /note="CC -> GG"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagca tccaccaagg
     1141 gcccatccgt cttccccctg gcgccctgct ccaggagcac ctccgagagc acagccgccc
     1201 tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg
     1261 ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga ctctactccc
     1321 tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac gaagacctac acctgcaacg
     1381 tagatcacaa gcccagcaac accaaggtgg acaagagagt tgagtccaaa tatggtcccc
     1441 catgcccacc ttgcccagca cctgagttcg aggggggacc atcagtcttc ctgttccccc
     1501 caaaacccaa ggacactctc atgatctccc ggacccctga ggtcacgtgc gtggtggtgg
     1561 acgtgagcca ggaagacccc gaggtccagt tcaactggta cgtggatggc gtggaggtgc
     1621 ataatgccaa gacaaagccg cgggaggagc agttcaacag cacgtaccgt gtggtcagcg
     1681 tcctcaccgt cctgcaccag gactggctga acggcaagga gtacaagtgc aaggtgtcca
     1741 acaaaggcct cgggtcctcc atcgagaaaa ccatctccaa agccaaaggg cagccccgag
     1801 agccacaggt gtacaccctg cccccatccc aggaggagat gaccaagaac caggtcagcc
     1861 tgacctgcct ggtcaaaggc ttctacccca gcgacatcgc cgtggagtgg gagagcaatg
     1921 ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac ggctccttct
     1981 tcctctacag caggctaacc gtggacaaga gcaggtggca ggaggggaat gtcttctcat
     2041 gctccgtgat gcatgaggct ctgcacaacc actacacaca gaagagcctc tccctgtctc
     2101 tgggtaaata aacagctagc ggttccaacc ttggttcccc tctccctttc ccccccctaa
     2161 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2221 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     2281 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2341 gaaggaagca gttcctctgg gagcttcttg aagacaaaca acgtctgtag cgaccctttg
     2401 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     2461 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     2521 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     2581 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     2641 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     2701 acgatgataa gcttgccaca acccgggatc ctctagagtc gacatggcca agttgaccag
     2761 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     2821 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     2881 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     2941 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     3001 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     3061 cgccctgcgc gacccggccg gcaactgcgt gcactttgtg gcagaggagc aggactgagc
     3121 ggccgcaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3181 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3241 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3301 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3361 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3421 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3481 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     3541 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3601 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3661 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcagatc
     3721 ccccgacctc gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3781 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc
     3841 ctcggagatc taagcttcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4801 gctgcaatga taccgcgtga cccacgctca ccggctccag atttatcagc aataaaccag
     4861 ccagccggaa gcgccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5641 cgcacatttc cccgaaaagt gccacctgaa gatctataca ttgaatcaat attggcaatt
     5701 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     5761 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccat
//
